Introduction: The study's objective was to assess how well cryotherapy reduces pain during arterio-venous fistula puncturing in hemodialysis patients.

Materials and Method: Thirty samples were chosen for the experimental group and thirty samples for the control group, totaling sixty samples. The Numerical Pain Rating Scale was used to gather the data. Cryotherapy was given to the experimental group. The post-test was administered without intervention to the control group. During the data collecting period, five to ten hemodialysis patients are evaluated every day. Both descriptive and inferential statistics were used to analyze the data.

Results: Following cryotherapy, there was a notable difference between the experimental and control groups. The degree of pain decreased. To link the post-test result to specific demographic characteristics, chi square analysis was performed. The post-test score and the experimental group's demographic characteristics are significantly correlated.

Conclusion: Arteriovenous fistula puncturing pain can be significantly decreased by cryotherapy.